Sodium Cacodylate Buffer

0.4M Sodium Cacodylate Buffer (250 mL)

0.4M Sodium Cacodylate = 21.4 g / 250 mL ddH2O

Weigh out 21.4 g Sodium Cacodylate and add to 200 mL H2O. Mix with a magnetic stirring rod. Adding about 8 mL of 0.2M HCl should adjust the pH to 7.2-7.4, however adding stronger HCl drop-by-drop and checking pH will also work. A target pH of 7.4 is better since the pH can lower slightly throughout processing. QS to 250 mL with ddH2O.