skip to main
|
skip to sidebar
EM Protocol Dump
Processing by Tissue
Wednesday, June 10, 2009
Making Resins
Here are some handy tables for mixing up various resins. Most people measure them out in weight, but we've found it easier to measure by volume. Lowicryl must be mixed with dry nitrogen bubbles and stored at -20C. Click to enlarge.
No comments:
Post a Comment
Newer Post
Older Post
Home
Subscribe to:
Post Comments (Atom)
Eric W. Roth
Northwestern University
View my complete profile
Search This Blog
Blog Archive
▼
2009
(35)
▼
June
(35)
2% GA in 0.05M PB, 0.1M Sucrose
2.5% GA, 4% PFA in 0.1M CB, 0.1M Sucrose
5% Gluteraldehyde in 0.1M Cacodylate Buffer
1% OsO4 in buffer
2% OsO4 in buffer
2% GA, 2% PFA in 0.1M Cacodylate Buffer
2% GA, 2% PFA in 0.1M Phosphate Buffer
Perfustion Fixative II
Standard processing for IEM
3% Paraformaldehyde for IEM
0.1M Cacodylate Buffer containing 4% Sucrose and 0...
0.1M Cacodylate Buffer containing 4% Sucrose IEM
Making Urynal Acetate and Lead Citrate
Postembedding Immunolabeling
Processing hard to penetrate tissues for standard TEM
Standard processing for TEM
Processing Platelets for Standard TEM
Platelet Resin
White's Buffer Solution
Glutaraldehyde in White's Saline
Muscle Processing for Standard TEM
0.18M Phosphate Buffer (muscle)
Isoosmotic Phosphate Buffer (Muscle)
0.1M Tris Wash (Muscle)
0.4M Phosphate buffer Stock (Muscle)
Mouse Glutaraldehyde Fixative (muscle)
1% OsO4 in 0.1M PB for Muscle
Toluidine Blue Stain
Sodium Phosphate Buffer from Monobasic and Dibasic...
Processing tissue for Immuno Electron Microscopy
Heavy Metal Staining for TEM Thin Sections
Making a loop for grid transfer
Making Resins
Sodium Cacodylate Buffer
Perfusion Fixative I
No comments:
Post a Comment