Making Urynal Acetate and Lead Citrate

Reynolds lead citrate

Place the following components into a volumetric flask:

1.33 g Lead nitrate
1.76 g sodium citrate
30 ml ddH2O

• Shake vigorously for 1 min and then intermittently for the next 30 minutes. Add 8 ml of 1M NaOH, at which time the solution becomes clear. Use only freshly made carbonate-free NaOH solution. Add ddH2O to the 50 ml mark and mix by inversion until the precipitate is dissolved.
• The pH should be about 12. It will keep for several months if kept stoppered.
• Aliquot into 1.5 mL eppendorf tube through a hydrophobic filter.
• Centrifuge @12k RPM for 3.5 min. before staining.

Uranyl Acetate (3% in 50% Methanol)

Can be made in a 15 mL tube.

0.42 g UA
14 mL 50% Methanol

• Mix and dissolve on a rotator 30 min.
• Store for 2 days @4C before use
• Aliquot into 1.5 mL eppendorf tube through a hydrophilic filter.
• Centrifuge @12k RPM for 3.5 min. before staining.