White's Buffer Solution

White’s Buffer Solution

White’s Solution A
Dissolve 14g NaCl (2.4M), 0.75g KCl (0.1M), 0.55g MgSO4 (46mM), 1.5g Ca(NO3)2.4H2O (64mM) in 100 mL ddH2O, and correct pH to 7.4. Store @ 4 oC

White’s Solution B
Dissolve 1.1g NaHCO3 (0.13M), 0.22 g Na2HPO4.7H2O (8.4mM), 0.05g anhydrous KH2PO4 (3.8mM), 0.01g phenol red in 100mL ddH2O, and correct pH to 7.4. Store @ 4 oC.