Wednesday, June 10, 2009

Toluidine Blue Stain


Materials:
Sodium Borate Tetra (EMS, CAT #21130)
Toludine Blue (EMS, Cat # 22050)
ddH20
150 mL beaker
Magnetic stir plate and bar
Hydrophylic filter

1. Wrap beaker with metal foil and combine the follow ingredients in order in a 150 mL beaker:
a) 1 g Sodium Borate Tetra
b) 100mL ddH2O
c) Stir until dissolved
d) 0.5-0.7 g Toludine Blue
e) Stir until dissolved (can be allowed to mix o/n), cover top so as to not let any light in

2. Filter prior to use
3. Store @RT

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