0.1M Cacodylate Buffer containing 4% Sucrose and 0.1M Glycine IEM

0.1M Cacodylate Buffer containing 4% Sucrose IEM

White's Buffer Solution

White’s Buffer Solution

White’s Solution A
Dissolve 14g NaCl (2.4M), 0.75g KCl (0.1M), 0.55g MgSO4 (46mM), 1.5g Ca(NO3)2.4H2O (64mM) in 100 mL ddH2O, and correct pH to 7.4. Store @ 4 oC

White’s Solution B
Dissolve 1.1g NaHCO3 (0.13M), 0.22 g Na2HPO4.7H2O (8.4mM), 0.05g anhydrous KH2PO4 (3.8mM), 0.01g phenol red in 100mL ddH2O, and correct pH to 7.4. Store @ 4 oC.

0.18M Phosphate Buffer (muscle)

Isoosmotic Phosphate Buffer (Muscle)

0.1M Tris Wash (Muscle)

0.4M Phosphate buffer Stock (Muscle)

Sodium Phosphate Buffer from Monobasic and Dibasic Stock

Here is a quick table for mixing up 0.1M Sodium Phosphate buffer from Monobasic and Dibasic stock solutions. To make it 0.2M, don't add water. Enjoy!