Showing posts with label sodium cacodylate. Show all posts
Showing posts with label sodium cacodylate. Show all posts
Thursday, June 11, 2009
Wednesday, June 10, 2009
Sodium Cacodylate Buffer
0.4M Sodium Cacodylate Buffer (250 mL)
0.4M Sodium Cacodylate = 21.4 g / 250 mL ddH2O
Weigh out 21.4 g Sodium Cacodylate and add to 200 mL H2O. Mix with a magnetic stirring rod. Adding about 8 mL of 0.2M HCl should adjust the pH to 7.2-7.4, however adding stronger HCl drop-by-drop and checking pH will also work. A target pH of 7.4 is better since the pH can lower slightly throughout processing. QS to 250 mL with ddH2O.
0.4M Sodium Cacodylate = 21.4 g / 250 mL ddH2O
Weigh out 21.4 g Sodium Cacodylate and add to 200 mL H2O. Mix with a magnetic stirring rod. Adding about 8 mL of 0.2M HCl should adjust the pH to 7.2-7.4, however adding stronger HCl drop-by-drop and checking pH will also work. A target pH of 7.4 is better since the pH can lower slightly throughout processing. QS to 250 mL with ddH2O.
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